Part:BBa_K277013

3L.3_23.A2.01

3L.3_23.A2.01 is 746 bases long and is cloned into the pGem-T vector.

3L.3_23.A2.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.01 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

loxP_site loxPsym_YCL061C (44..77)

gene YCL061C (81..+3371) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint

Sequence (the first 746 bases correspond to coordinates 8871..9616 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 376
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 376
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 376
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 376
  
• 1000
  COMPATIBLE WITH RFC[1000]